at area temperature. After washing in PBS, the sections had been incubated for 30minutes at area temperature with an FITC conjugated goat anti rabbit IgG, then washed in PBS and counterstained with 40 six diamidino 2 phenylindole nuclear stain. Laser scanning confocal microscopy was carried out using an FV 1000/ES confocal microscope.
Showing posts with label Cannabinoid Receptor. Show all posts
Showing posts with label Cannabinoid Receptor. Show all posts
Thursday, August 23, 2012
EKB-569 Widespread Myths Compared To The Actual Details
The four mm sections of mouse ear had been fixed in 10% formalin in PBS and stained with hematoxylin and eosin. Frozen sections of mouse ear had been prepared for immunofluorescence staining. Sections had been fixed with precooled acetone for 10 minutes at area temperature, blocked for 30minutes at area temperature with 5% goat serum in PBS, and incubated with rabbit anti mouse K17 pAb for 1 hour at area temperature. After washing in PBS, the sections had been incubated for 30minutes at area temperature with an FITC conjugated goat anti rabbit IgG, then washed in PBS and counterstained with 40 six diamidino 2 phenylindole nuclear stain. Laser scanning confocal microscopy was carried out using an FV 1000/ES confocal microscope.
at area temperature. After washing in PBS, the sections had been incubated for 30minutes at area temperature with an FITC conjugated goat anti rabbit IgG, then washed in PBS and counterstained with 40 six diamidino 2 phenylindole nuclear stain. Laser scanning confocal microscopy was carried out using an FV 1000/ES confocal microscope.
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